AN IMPROVED COLORIMETRIC METHOD FOR THE QUANTITATIVE ESTIMATION OF THYMINE

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An improved colorimetric method for the quantitative estimation of thymine.

The Hunter (1) procedure was originally developed for the calorimetric d&&ion of minute amounts of thymine. In this procedure thymine is coupled under air with p-diazobenzenesulfonic acid in carbonate solut.ion, and a red color is subsequently produced by the addition of sodium hydroxide and hydroxylamine. Thymine was determined by this means in hydrolysates of dcsoxypentose nucleic acids (DNA)...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1952

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)55671-4